Collecting and Storing Tissue From Young Patients With Cancer



Status:Completed
Conditions:Ovarian Cancer, Liver Cancer, Liver Cancer, Cancer, Cancer, Brain Cancer, Brain Cancer, Brain Cancer, Blood Cancer, Blood Cancer, Blood Cancer, Blood Cancer, Blood Cancer, Infectious Disease, Lymphoma, Hematology, Hematology
Therapuetic Areas:Hematology, Immunology / Infectious Diseases, Oncology
Healthy:No
Age Range:Any - 21
Updated:8/9/2018
Start Date:March 5, 2007

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Establishing Continuous Cell Lines and Xenografts From Pediatric Cancers for Biological and Pre-Clinical Therapeutic Studies

This laboratory study is collecting and storing tissue, blood, and bone marrow samples from
young patients with cancer. Collecting and storing samples of tissue, blood, and bone marrow
from patients with cancer to study in the laboratory may help doctors learn more about
changes that may occur in DNA and identify biomarkers related to cancer.

PRIMARY OBJECTIVES:

I. Establish and bank cell lines and/or xenografts from pediatric patients with cancer.

II. Establish continuous cell lines, under carefully controlled conditions, from pediatric
patients with cancer.

III. Establish transplantable xenografts in immunocompromised mice from tumor cells that are
difficult to establish as continuous cell lines in vitro.

IV. Create a bank of cell lines and generate sufficient vials of cryopreserved cells for
distribution to investigators with approved COG biology protocols. V. Characterize cell lines
from childhood cancers with respect to DNA short tandem repeat molecular profile as a
?fingerprint? of original cell line identity.

VI. Characterize cell lines for the ability for sustained growth in tissue culture and/or as
mouse xenografts.

VII. Characterize cell lines for mycoplasma contamination. VIII. Characterize cell lines for
expression of molecular makers that confirm the tumor-type of the cell line and the immortal
nature of the cells (telomerase) and the expression of molecular markers that may correlate
with drug resistance.

OUTLINE: This is a multicenter study.

Specimens are stratified according to disease (acute lymphoblastic leukemia vs acute myeloid
leukemia vs lymphoma vs osteogenic sarcoma vs Ewing family of tumors vs rhabdomyosarcoma vs
primitive neuroectodermal tumor vs glioma vs astrocytoma vs rhabdoid tumors vs hepatoblastoma
vs retinoblastoma vs Wilms tumor vs germ cell tumors vs other diagnoses).

Leftover tissue from diagnostic procedures and/or surgery is cryopreserved and banked. Blood
and/or bone marrow are also collected and banked. Cell lines are established and
characterized via reverse-transcriptase polymerase chain reaction and/or flow cytometry for
biomarkers and by DNA fingerprinting. Markers to be identified may include the following:

NEUROBLASTOMA: tyrosine hydroxylase, protein gene product (PGP) 9.5, GD2, HLA class I, and
HSAN 1.2 antigens

EWING FAMILY OF TUMORS: EWS-FLI1, EWS-ERG, and PGP 9.5

RETINOBLASTOMA: interphotoreceptor retinoid-binding protein

ACUTE LYMPHOBLASTIC LEUKEMIA: immunophenotype

ALVEOLOR RHADOMYOSARCOMA: PAX3-FKHR, PAX7-FKHR, and MyoD1

ALL CELL TYPES: telomerase expression including hTR and hTERTMutations of TP53 gene are
detected by flow cytometry and/or immunocytochemistry.

No results of these tests are provided to the patient, the patient's physician, or the
patient's medical records.

Inclusion Criteria:

- All malignant tissues from childhood cancers allowed including the following:

- Brain tumors (all types)

- Tissue should be submitted to CNS Committee Resource labs to be forwarded
for this study, unless instructed otherwise on the COG web site

- Ewing family of tumors

- Rhabdomyosarcomas

- Other soft tissue sarcomas

- Osteogenic sarcomas

- Rhabdoid tumors

- Neuroblastomas

- Viable material for cell culture for neuroblastoma is collected via
COG-ANBL00B1 and should not be submitted via this study unless the patient
cannot be enrolled on COG-ANBL00B1*

- Retinoblastomas

- Anaplastic Wilms tumor

- Germ cell tumors

- Leukemias/lymphomas

- Acute myeloid leukemia (AML)

- Blood samples and bone marrow samples from patients at second relapse
and beyond may be submitted for this study

- Bone marrow samples at diagnosis or first relapse must be submitted to
an AML resource lab and will be forwarded for this study at the
discretion of the AML Committee

- Acute lymphoblastic leukemia (ALL)

- Blood samples may be submitted directly to this study

- Bone marrow samples must be submitted to an ALL resource lab and will
be forwarded for this study at the discretion of the ALL Committee

- Enrolled on a COG therapeutic, biology, or tissue banking protocol that allows
collection of tissue for research and submission to a COG-designated resource
laboratory

- Participation in this protocol is not permitted until after tissue requirements
for any active COG disease-specific therapeutic, biology, or banking protocols
have been satisfied

- Material may only be submitted for this protocol if tissue is available in excess
of that required for satisfying active disease-specific therapeutic and
biological protocols

- Patients with diagnosis pending are eligible
We found this trial at
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1 Tampa General Cir
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1600 7th Avenue
Birmingham, Alabama 35233
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Children's Hospital of Alabama Children
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11100 Euclid Avenue
Cleveland, Ohio 44106
(216) 844-1000
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Rainbow Babies and Children's Hospital UH Rainbow Babies & Children’s Hospital is a 244-bed, full-service...
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3533 South Alameda Street
Corpus Christi, Texas 78411
(361) 694-5000
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Driscoll Children's Hospital Driscoll Children's Hospital was built because Clara Driscoll's will requested that a...
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1500 E Duarte Rd
Duarte, California 91010
(626) 256-4673
Phone: 800-826-4673
City of Hope Comprehensive Cancer Center City of Hope is a leading research and treatment...
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9981 S Healthpark Dr # 465
Fort Myers, Florida 33908
239-343-5000
Lee Memorial Health System Our origins can be traced to the Fall of 1916 when...
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282 Washington St
Hartford, Connecticut 06106
(860) 545-9000
Phone: 860-545-9967
Connecticut Children's Medical Center Connecticut Children’s Medical Center is a nationally recognized, 187-bed not-for-profit children’s...
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524 South Park Street
Kalamazoo, Michigan 49007
(269) 341-7654
Phone: 269-341-6350
Bronson Methodist Hospital Our healthcare system serves patients and families throughout southwest Michigan and northern...
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2401 Gillham Rd
Kansas City, Missouri 64108
(816) 234-3000
Phone: 816-234-3265
Children's Mercy Hospital Children's Mercy Hospitals and Clinics continues redefining pediatric medicine throughout the Midwest...
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1800 West Charleston Boulevard
Las Vegas, Nevada 89102
(702) 383-2000
University Medical Center of Southern Nevada University Medical Center is dedicated to providing the highest...
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529 West Markham Street
Little Rock, Arkansas 72205
(501) 686-7000
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4650 Sunset Blvd
Los Angeles, California 90027
 (323) 660-2450
Childrens Hospital Los Angeles Children's Hospital Los Angeles is a 501(c)(3) nonprofit hospital for pediatric...
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4015 22nd Place
Lubbock, Texas 79410
806-725-0000
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Covenant Children's Hospital Every child is different. And when they're sick or injured, they deserve...
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747 52nd St
Oakland, California 94609
(510) 428-3000
Children's Hospital and Research Center Oakland For nearly 100 years, Children's Hospital & Research Center...
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530 Northeast Glen Oak Avenue
Peoria, Illinois 61603
(309) 624-4945
Phone: 309-655-4242
Saint Jude Midwest Affiliate The Jim and Trudy Maloof St. Jude Midwest Affiliate Clinic was...
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4401 Penn Avenue
Pittsburgh, Pennsylvania 15224
412-692-5325
Phone: 412-692-7056
Children's Hospital of Pittsburgh of UPMC UPMC is one of the leading nonprofit health systems...
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7700 Floyd Curl Dr
San Antonio, Texas 78229
(210) 575-7000
Phone: 210-575-7268
Methodist Children's Hospital of South Texas Methodist Children
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4502 Medical Drive
San Antonio, Texas 78284
(210) 567-7000
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2401 W Belvedere Ave
Baltimore, Maryland 21215
(410) 601-9000
Sinai Hospital of Baltimore Sinai Hospital of Baltimore provides a broad array of high-quality, cost-effective...
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100 E Idaho St
Boise, Idaho 83712
(208) 381-2711
Saint Luke's Mountain States Tumor Institute For more than 100 years, St. Luke
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55 Fruit St
Boston, Massachusetts 02114
(617) 724-4000
Massachusetts General Hospital Cancer Center An integral part of one of the world
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450 Brookline Ave
Boston, Massachusetts 2215
617-632-3000
Dana-Farber Cancer Institute Since it’s founding in 1947, Dana-Farber has been committed to providing adults...
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Charlotte, North Carolina 28204
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1200 West Harrison Stree
Chicago, Illinois 60607
(312) 996-4350
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2049 E 100th St
Cleveland, Ohio 44106
(216) 444-2200
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Columbia, Missouri 65201
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Dayton, Ohio 45404
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4160 John R St #2122
Detroit, Michigan 48201
(313) 833-1785
Wayne State University/Karmanos Cancer Institute Karmanos is based in southeast Michigan, in midtown Detroit, and...
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9333 Imperial Highway
Downey, California 90242
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900 West Faris Rd.
Greenville, South Carolina 29605
(864)455-8898
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BI-LO Charities Children's Cancer Center The BI-LO Charities Children
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Greenville, South Carolina 29605
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30 Prospect Ave
Hackensack, New Jersey 07601
(201) 996-2000
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Kalamazoo, Michigan 49008
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2018 W Clinch Ave
Knoxville, Tennessee 37916
(865) 541-8000
Phone: 865-541-8266
East Tennessee Children's Hospital East Tennessee Children's Hospital is a not-for-profit, private, independent pediatric medical...
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Las Vegas, Nevada 89109
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Las Vegas, Nevada 89144
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601 South Rancho Drive Suite C-26
Las Vegas, Nevada 89106
(702) 384-0013
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1 Children's Way
Little Rock, Arkansas 72202
(501) 364-1100
Arkansas Children's Hospital Arkansas Children's Hospital (ACH) is the only pediatric medical center in Arkansas...
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Louisville, Kentucky 40202
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Lubbock, Texas 79430
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Morristown, New Jersey 07962
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New Orleans, Louisiana 70121
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1430 Tulane Ave Suite SL32
New Orleans, Louisiana 70112
(504) 588-5912
Tulane University Health Sciences Center One of the nation's most recognized centers for medical education,...
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940 NE 13th St
Oklahoma City, Oklahoma 73190
(405) 271-6458
University of Oklahoma Health Sciences Center The OU Health Sciences Center is composed of seven...
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8200 Dodge St
Omaha, Nebraska 68114
(402) 955-5400
Children's Hospital and Medical Center of Omaha Children's Hospital & Medical Center has a rich...
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Emile St
Omaha, Nebraska 68198
(402) 559-4000
Univ of Nebraska Med Ctr A vital enterprise in the nation’s heartland, the University of...
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Saint Petersburg, Florida 33701
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801 N Rutledge St
Springfield, Illinois 62702
(217) 545-8000
Southern Illinois University School of Medicine At SIU School of Medicine, research includes biologically oriented...
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99 Beauvoir Avenue
Summit, New Jersey 07902
(908) 522-2000
Overlook Hospital Atlantic Health System
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9040 Jackson Ave
Tacoma, Washington 98431
(253) 968-1110
Madigan Army Medical Center Located on Joint Base Lewis-McChord, Madigan Army Medical Center comprises a...
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Vancouver, British Columbia
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901 45th St
West Palm Beach, Florida 33407
(561) 844-6300
Saint Mary's Hospital Our team of dedicated physicians, nurses and staff offer a broad spectrum...
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1 Medical Center Blvd
Winston-Salem, North Carolina 27157
336-716-2011
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