Detection of Either the EML4-ALK Gene Rearrangements or the T790M EGFR Mutation in the Plasma of Advanced NSCLC Patients

The goal of this study is two-fold: 1) Demonstrate feasibility of detection of EML4-ALK
fusion transcripts in the circulation of NSCLC patients positive for ALK on tissue analysis,
using an institutionally accepted assay; 2) Demonstrate feasibility of detection of T790M
EGFR mutation in the circulation of NSCLC patients positive for T790M on tissue analysis,
using an institutionally accepted assay.

Inclusion Criteria:

1. Participants must have histologically confirmed NSCLC, stage IIIB- IV, and have tested
positive for the presence of EML4-ALK on tissue specimen, fresh or archived, using an
institutionally accepted assay.

2. Age of 18 years or older.

3. Participants are being considered for ALK-directed therapy or have progressed on EGFR
TKI treatment.

4. Participants must have hemoglobin > 10 mg/dL.

5. Ability to understand and willingness to sign a written informed consent document.

Exclusion Criteria:

1. Hepatitis (all types) in patient's medical record

2. HIV documented in patient's medical record

3. Hemoglobin < 10 mg/dL

4. Less than 18 years of age

5. Histologically confirmed NSCLC Stage I-IIIA

6. Tested negative for the presence of EML4-ALK and T790M EGFR on tissue