ATG-GCSF in New Onset Type 1 Diabetes

The primary statistical hypothesis to be assessed in the study is whether the 2 hour area
under the curve (change in baseline to 12 months) in residual beta cell function (C-peptide)
will differ between those treated with ATG and GCSF or ATG alone as compared with placebo.

The study will also examine the effect of the proposed treatments on surrogate markers for
immunologic and metabolic outcomes.

Inclusion Criteria:

- Must be > 12 years < 46

- Must have a diagnosis of T1D for less than 100 days at randomization

- Willing to provide Informed Consent or have a parent or legal guardian provide
informed consent if the subject is <18 years of age

- Positive for at least one islet cell autoantibody; glutamic acid decarboxylase 65
(GAD65A), Insulin micro IAA (mIAA), if obtained within 10 days of the onset of insulin
therapy, islet antigen 2 (IA-2A), Islet Cell Antigen (ICA), or zinc transporter 8
(ZnT8A)

- Must have stimulated C-peptide levels = 0.2 pmol/ml measured during a mixed meal
tolerance test (MMTT) conducted at least 21 days from diagnosis of diabetes and within
one month (37 days) of randomization

- Must be Epstein-Barr virus (EBV PCR) negative within two weeks of randomization if EBV
seronegative at screening

- Be at least 6 weeks from last live immunization

- Participants are required to receive killed influenza vaccination at least 2 weeks
prior to randomization when vaccine for the current or upcoming flu season is
available

- Be willing to forgo vaccines during the treatment period and for 3 months following
last dose of study drug

- Be willing to comply with intensive diabetes management

Exclusion Criteria:

- Be immunodeficient or have clinically significant chronic lymphopenia: (Leukopenia (<
3,000 leukocytes /µL), neutropenia (<1,500 neutrophils/µL), lymphopenia (<800
lymphocytes/µL), or thrombocytopenia (<100,000 platelets/µL).

- Have active signs or symptoms of acute infection at the time of randomization

- Have evidence of prior or current tuberculosis infection as assessed by purified
protein derivative (PPD), interferon gamma release assay (IGRA) or by history

- Be currently pregnant or lactating, or anticipate getting pregnant within the two year
study period

- Require use of other immunosuppressive agents including chronic use of systemic
steroids

- Have evidence of current or past human immunodeficiency virus (HIV), Hepatitis B or
Hepatitis C infection

- Have any complicating medical issues or abnormal clinical laboratory results that may
interfere with study conduct, or cause increased risk to include pre-existing cardiac
disease, chronic obstructive pulmonary disease (COPD), sickle cell disease,
neurological, or blood count abnormalities

- Have a history of malignancies other than skin

- Evidence of liver dysfunction with aspartate aminotransferase (AST) or alanine
transaminase (ALT) greater than 3 times the upper limits of normal

- Evidence of renal dysfunction with creatinine greater than 1.5 times the upper limit
of normal

- Vaccination with a live virus within the last 6 weeks

- Current or ongoing use of non-insulin pharmaceuticals that affect glycemic control
within prior 7 days of screening

- Active participation in another T1D treatment study in the previous 30 days

- Prior treatment with abatacept or anti-cd3

- Known allergy to GCSF or ATG

- Prior treatment with ATG or known allergy to rabbit derived products

- Any condition that in the investigator's opinion may adversely affect study
participation or may compromise the study results