Autologous Umbilical Cord Blood Infusion for Children With Autism Spectrum Disorder (ASD)

Autism Spectrum Disorder (ASD) is a neurodevelopment disorder with early onset in life.
Currently, available treatments for patients with ASD are supportive, but not curative.
Umbilical cord blood (UCB) has been shown to lessen the clinical and radiographic impact of
hypoxic brain injury and stroke in animal models and in infants with hypoxic ischemic
encephalopathy. UCB also engrafts and differentiates in the brain, facilitating neural cell
repair in animal models and human patients with inborn errors of metabolism undergoing
allogeneic, unrelated donor UCB transplantation. Infusion of autologous UCB does not require
immunosuppression and has been shown to be safe in young children with brain injuries such as
cerebral palsy and stroke. In this study, the investigators hypothesize that infusion of a
patient's own umbilical cord blood cells (UCB) can offer neural protection/repair in the
brain and reduction of inflammation associated with this disorder.

Inclusion Criteria:

1. Age ≥ 24 months to ≤72months at the time of visit 1

2. Confirmed clinical DSM-5 diagnosis of Autism Spectrum Disorder using all three of the
following measures:

- Autism Diagnostic Observation Schedule - Toddler or Generic (ADOS)

- Autism Diagnostic Interview-Revised (ADI-R)

- DSM-5 checklist

3. IQ ≥ 35 on Stanford Binet Intelligence Scale or similar standardized test

4. Autologous umbilical cord blood available from a cord blood bank with a minimum total
nucleated cell dose of ≥ 1 x 107 cells/kilogram of subject weight that meets
acceptance criteria outlined in section 6.0 with confirmed HLA matching

5. Stable on current medications for at least 2 months prior to infusion of cord blood

6. Ability to travel to Duke University three times (0, 6, 12 mo.), parent/guardian able
to participate in electronic communication tracking two times in the study and interim
phone surveys every 3 months

7. Parental consent

8. Subject and parent/guardian must be English speaking

Exclusion Criteria:

1. Unwilling to commit to follow up for a year

2. History of prior cell therapy

3. Use of IVIG or other anti-inflammatory medications with the exception of NSAIDs

4. Medical records indicate that child has genetic or other syndromes such as fragile X,
neurofibromatosis, Rett syndrome, tuberous sclerosis, PTEN mutation, cerebral palsy,
cystic fibrosis, muscular dystrophy, Crohn's disease, or rheumatoid disease

5. Co-morbid condition that would influence child's performance on assessments.

6. Central Nervous System (CNS) infection

7. History of unstable epilepsy or uncontrolled seizure disorder, infantile spasms,
Lennox Gastaut syndrome, Dravet syndrome

8. Known pathogenic copy number variation (CNV) (e.g. 16p11.2, 15q13.2, 2q13.3)

9. Significant sensory (i.e., deafness, blind) or motor impairment (CP) (if using
Language Environment Analysis (LENA), no uncorrected hearing impairment)

10. Presence of obvious physical dysmorphology

11. Review of medical records indicates ASD diagnosis not likely or other serious
complicating genetic or medical condition present

12. Impaired renal or liver function as determined by serum creatinine >1.5mg/dL and/or
total bilirubin>1.3mg/dL

13. Clinically significant abnormalities in Complete Blood Count (CBC): Hemoglobin < 10.0
g/dL, White Blood Count (WBC) < 3.8 x 10e9, Platelets < 150x 10e9.

14. Known metabolic disorder, mitochondrial dysfunction

15. Uncontrolled infection, presence of or infection with HIV

16. Active malignancy

17. Macroencephaly or microencephaly ( >2 standard deviations in the relevant direction
between head circumference and height)

18. Change in current stable use of psychoactive medications; as per parent report.