In 1897, the university moved from Forty-ninth Street and Madison Avenue, where it had stood for forty years, to its present location on Morningside Heights at 116th Street and Broadway. Seth Low, the president of the University at the time of the move, sought to create an academic village in a more spacious setting. Charles Follen McKim of the architectural firm of McKim, Mead, and White modeled the new campus after the Athenian agora. The Columbia campus comprises the largest single collection of McKim, Mead & White buildings in existence.

In addition to its New York City campuses, Columbia has two facilities outside of Manhattan. Nevis Laboratories, established in 1947, is Columbia's primary center for the study of high-energy experimental particle and nuclear physics. Located in Irvington, New York, Nevis is situated on a 60-acre estate originally owned by the son of Alexander Hamilton.

The Lamont-Doherty Earth Observatory was established in 1949 in Palisades, New York, and is a leading research institution focusing on global climate change, earthquakes, volcanoes, nonrenewable resources, and environmental hazards. It examines the planet from its core to its atmosphere, across every continent and every ocean.