Electrical Stimulation of Human Myocytes in Microgravity

Inclusion Criteria:

- Participant is capable of giving informed consent and has voluntarily signed and dated
an informed consent form, approved by an Institutional Review Board and provided
Health Insurance Portability and Accountability Act authorization (HIPAA) or other
privacy authorization prior to any participation in study.

- Participant is 20-40 or 60-80 years of age, inclusive

- Participant BMI <30 kg/m2

- Participant states willingness to follow protocol as described, including the
prescribed activity level and completing any forms needed throughout the study.

Exclusion Criteria:

- Participant reports a history of type 1 or type 2 Diabetes; or Hgb A1c > 6.5% at
screening visit.

- Participant is actively pursuing weight loss and/or lifestyle changes.

- Participant has a history of pressure ulcers.

- Participant has a stated history of Deep Vein Thrombosis (DVT), recent (within last 3
months) pulmonary embolism, or has a positive D-dimer test and lower extremity
ultrasound at screening, or a known hypercoagulable condition, or other clotting or
bleeding disorders.

- Participant has poorly controlled hypertension or hypotension in the opinion of the
study PI or medical investigator (MI).

- Participant has untreated hypothyroidism or hyperthyroidism.

- Participant has current infection (requiring prescription antimicrobial or antiviral
medication, or hospitalization), or corticosteroid treatment (with the exception of
inhaled or topical steroids) in the last 3 months prior to screening visit.

- Participant is currently taking prescription strength anti-inflammatory medication or
has taken prescription strength anti-inflammatory medication in the 6 weeks prior to
screening.

- Participant has had surgery requiring >2 days of hospitalization in the last 3 weeks
prior to screening visit.

- Participant has an active malignancy or autoimmune disease.

- Participant has current significantly impaired liver function in the opinion of the
study PI (mild asymptomatic fatty liver is acceptable), or hepatic enzyme tests are
≥2.5 times normal limit.

- Participant has a chronic, contagious, infectious disease, such as active
tuberculosis, Hepatitis B or C, or HIV, per self-report.

- Participant is an amputee and/or has presence of partial or full artificial limb.

- Participant has had a significant cardiovascular event (e.g. myocardial infarction,
stroke) ≤ 6 months prior to screening visit; or stated history of congestive heart
failure; or participant has evidence of cardiovascular disease assessed during the ECG
at screening.

- Participant has a history of, or currently has uncontrolled severe diarrhea, nausea or
vomiting.

- Participant has an obstruction of the gastrointestinal tract, inflammatory bowel
disease, short bowel syndrome or other forms of gastrointestinal disease such as stage
III or above gastroesophageal reflux disease, gastroparesis, peptic ulcer disease,
celiac disease, intestinal dysmotility, diverticulitis, ischemic colitis.

- Participant has used tobacco within the last 3 months.

- Participant has a history of drug or alcohol abuse (> 3 drinks per day) within the
last 5 years

- Female participant is pregnant, lactating or is within 6 weeks postpartum prior to the
screening visit

- Participant has impaired renal function: estimated glomerular filtration rate (eGFR) ≤
50 ml/min/1.73m2 determined at screening.

- Participant's triglyceride level is ≥ 250 mg/dl and LDL cholesterol is > 150 mg/dl at
screening.

- Participant has a urine albumin-to-creatinine ratio (UACR) ≥300 mg/g at screening in a
single urine specimen (per National Kidney Foundation guideline).