Effect of Topical Sulforaphane on Skin Aging and With Ultraviolet and Visible Light Exposure

The study is to compare the expression of both keratin 16 and 17 before and after application
of sulforaphane on both photoprotected and photoexposed area, and to determine whether these
findings alter skin aging as well as skin response to ultraviolet (UV) and visible light
exposure.

Each study participant will have up to eight study visits. Topical sulforaphane will be
applied for up to 6 months. Photography, clinical assessment, UV light irradiation, Visible
light treatment, Skin biopsies, non invasive elasticity measurements such as cutometer and
ballistometer, and others will be involved in this study.

Inclusion Criteria:

- Participants must be over the age of 18 years old with healthy skin or moderate degree
of photoaging/intrinsic aging;

- Must be healthy enough to undergo skin biopsy, light irradiation, and other study
procedures in the opinion of the investigator;

- Must be willing to comply with the requirements of the protocol;

- Must have the ability to understand and communicate with the investigator;

- Participant must provide informed consent.

Exclusion Criteria:

- Subjects who are unable to provide informed consent;

- Subject with significant medical history or current skin diseases that the
investigator feels is not safe for study participation;

- Subjects who have been treated with systemic retinoids or steroids within the past
month prior to entry to the study;

- Subjects who have been treated with topical steroids, retinoids or other topical drugs
used within 2 weeks prior to entry to the study;

- Recently treated or current skin diseases that would affect clinical evaluation and
biopsy;

- Subjects with a known allergy to broccoli.

- Presence or suspicion of bleeding disorder or diathesis which would complicate biopsy.

- Subjects with a history of excessive scar or keloid formation in the past 10 years.

- Pregnant or nursing subjects (self-reported).

- Subjects with known allergy to anesthetics used.

- Patients with history of investigational drug use in the 30 days prior to entry into
the study.