Fresh Vs Frozen Surgical Sperm in IVF

The study seeks to isolate the effect of slow freezing and thawing of surgical sperm
specimens on fertilization and embryo blastulation rates by utilizing a randomized controlled
split cohort protocol including men with obstructive azoospermia undergoing surgical sperm
retrieval and good-prognosis female patients.

Inclusion Criteria:

- Females age < 42 with an indication for IVF

- Females with lowest AMH level 1.2 ng/mL or higher

- Females with FSH < 13 mIU/mL

- Sperm obtained via Surgical retrieval (any type) for IVF only

- Use of comprehensive chromosome screening (CCS) to screen embryos for aneuploidy

- BMI < 35

- Baseline antral follicle count of > 8

- Single embryo transfers only

Exclusion Criteria:

- Anything that would place the individual at increased risk or preclude the
individual's full compliance with or completion of the study.

- Contraindication to IVF

- Clinical indication for preimplantation genetic diagnosis (PGD) (i.e., screening for
single gene disorder, chromosomal translocation, or any other disorders requiring
detailed embryo genetic analysis)

- Fewer than 4 mature oocytes retrieved (will not randomize)

- Male partner with non-obstructive azoospermia

- Male partner with any Karyotype other than 46,XY

- Male partner with history of spinal cord injury

- Male partner with Kallman's syndrome

- History of chronic oligomenorrhea

- History of hydrosalpinges or adnexal mass

- History of endometrial insufficiency (max endometrial thickness < 7mm)