Mechanisms of Exacerbation of Asthma

In this study, we will explore mechanisms of exacerbation in asthma by careful
characterization studies in asthmatics presenting in acute severe exacerbation. Specifically,
we will determine the frequency of secretor status in these subjects by blood and saliva
testing, and we will also perform a detailed cellular and biochemical analysis of their
airway secretions. We will collect airway secretions using two methods. One method will be to
have subjects cough sputum into a clean plastic container. The other method only applies to
asthmatics with near-fatal asthma requiring intubation and mechanical ventilation. In these
asthmatics, we will collect airway secretions by tracheal aspirate using methods normally
applied in their clinical care.

Inclusion Criteria:

1. Male and female subjects with a history of asthma between the ages of 18 to 75 years.

2. Physician diagnosis of asthma.

3. Currently experiencing an acute exacerbation of asthma. This exacerbation may be
severe necessitating treatment in the emergency room, ICU, or hospital ward.

4. Ability to provide informed consent.

Exclusion Criteria:

1. Lung disease other than asthma.

2. Females who are lactating or who are pregnant.

3. Use of recreational drugs in the 1 month preceding the study.