Exercise Changes to Peripheral Blood Mononuclear Cells in Children
| Status: | Completed |
|---|---|
| Conditions: | Obesity Weight Loss |
| Therapuetic Areas: | Endocrinology |
| Healthy: | No |
| Age Range: | 8 - 18 |
| Updated: | 4/2/2016 |
| Start Date: | September 2004 |
| End Date: | February 2015 |
| Contact: | Lori D. Wilson, PhD |
| Email: | lwilson@uci.edu |
| Phone: | 714-456-2246 |
PBMC, Exercise and Children: Initial Mechanisms
The goal of this research is to determine how the peripheral immune system is altered by
exercise and differences related to gender, pubertal status and health.
exercise and differences related to gender, pubertal status and health.
SPECIFIC AIMS:
1. To systematically measure for the first time in healthy children and adolescents the
effects of brief bouts of exercise on:
1. Numbers of circulating PMBCs, their subsets and key intercellular adhesion
molecules (ICAMs).
2. PBMC gene regulation of stress, inflammatory, and growth/repair mediators
[including: interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor-alpha
(TNF-alpha), interferon-gamma), growth hormone (GH), insulin-like growth factor-I
(IGF-I), heat shock proteins (Hsp)].
3. Circulating (serum) and intracellular PBMC levels of key mediators by flow
cytometry and cell culture techniques.
4. Circulating endogenous triggers of PBMC mediator responses—soluble Hsp, IL-6, and
F2-isoprostanes.
2. To determine how the acute PBMC responses are altered by gender, pubertal status, body
composition (measured by whole-body and regional DEXA), and fitness (measured by
progressive cycle ergometry and gas exchange).
3. To determine the relationship in healthy children and adolescents among acute PBMC
responses to exercise, biochemical precursors of the metabolic syndrome (insulin,
glucose, lipids), and the balance of the TH1/TH2 immune response.
1. To systematically measure for the first time in healthy children and adolescents the
effects of brief bouts of exercise on:
1. Numbers of circulating PMBCs, their subsets and key intercellular adhesion
molecules (ICAMs).
2. PBMC gene regulation of stress, inflammatory, and growth/repair mediators
[including: interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor-alpha
(TNF-alpha), interferon-gamma), growth hormone (GH), insulin-like growth factor-I
(IGF-I), heat shock proteins (Hsp)].
3. Circulating (serum) and intracellular PBMC levels of key mediators by flow
cytometry and cell culture techniques.
4. Circulating endogenous triggers of PBMC mediator responses—soluble Hsp, IL-6, and
F2-isoprostanes.
2. To determine how the acute PBMC responses are altered by gender, pubertal status, body
composition (measured by whole-body and regional DEXA), and fitness (measured by
progressive cycle ergometry and gas exchange).
3. To determine the relationship in healthy children and adolescents among acute PBMC
responses to exercise, biochemical precursors of the metabolic syndrome (insulin,
glucose, lipids), and the balance of the TH1/TH2 immune response.
Inclusion Criteria:
- appropriate Tanner Stage
- No evidence of disease or disability
Exclusion Criteria:
- no use of antiinflammatory medications, alcohol, illegal drugs or bronchodilators
- elite children participating in extensive exercise or dance programs
- pregnant
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