AT-001 for Long-term Preservation of Brain Health in Aging

Double blind, placebo-controlled, single-center study. Approximately 40 healthy volunteers
will be randomized to the study drug and 20 healthy volunteers randomized to the placebo, for
a total of 60 enrolled participants.

All participants will be treated with the study drug or placebo for 12 months. Subjects will
be seen in the clinic for the following visits: Screening (Day -14), Baseline (Day 0), Month
6, and End-of-Study (Month 12) while on study drug. An additional safety visit at Month 13
(four weeks after study drug discontinuation) will be required of all participants.

Participants will undergo vital signs assessment, medical history review, medication list
review, and review of Adverse Events (AEs) and Serious Adverse Events (SAEs) at each visit.

Participants will undergo neuropsychological testing and routine physical examinations at the
screening, baseline, and end-of-study visits.

Participants will undergo blood draws, urine collection, MRI to assess lumbar puncture
safety, and lumbar puncture at the baseline and end-of-study visits.

Inclusion Criteria:

- Not demented by DSM-IV criteria.

- English-speaking, to ensure compliance with cognitive testing and study visit
procedures.

- Montreal Cognitive Assessment (MoCA) score at screening visit ≥24/30.

- Female participants must not be pregnant or of childbearing potential (surgically
sterile or post-menopausal for >1 year).

- Stable medical condition for 3 months prior to screening visit in the opinion of the
study physician.

- Non-diabetic, confirmed by fasting serum glucose <120 mg/dL and on no oral
hypoglycemic agents or insulin treatment.

- No other clinically significant abnormalities on labs (CBC, chemistry panel, PT/INR,
aPTT, urinalysis).

- Stable medications for 4 weeks prior to screening.

- Able to ingest oral medications.

- Physically acceptable for the study as confirmed by medial history, physical exam,
neurological exam, and clinical tests in the opinion of the study physician.

Exclusion Criteria:

- Significant neurological disease, such as Parkinson's disease, brain tumor, multiple
sclerosis, or seizure disorder

- Major depression in the past 12 months (DSM-IV criteria), major mental illness such as
schizophrenia, or recent (in past 12 months) alcohol or substance abuse.

- History of invasive cancer within the past 2 years (excluding non-melanoma skin
cancer).

- Contraindications to lumbar puncture (bleeding disorder, platelet count <100,000,
anticoagulant treatment, major structural abnormality or sepsis in the area of the
lumbosacral spine, or previous lower back surgery that would make LP technically
difficult in the opinion of the study physician, hypersensitivity to lidocaine).

- Contraindications to MRI (pacemaker, shrapnel, metallic implants, etc).

- Clinically significant abnormalities on labs (CBC, chemistry panel, PT/INR, aPTT,
urinalysis).

- Use of any investigational agents within 30 days prior to screening.

- Major surgery within eight weeks prior to the Baseline Visit.

- Severe unstable major medical illnesses, including uncontrolled cardiac conditions or
heart failure (New York Heart Association Class III or IV).

- Antiretroviral therapy for human immunodeficiency virus (HIV).

- Residence in a skilled nursing facility.

- Blindness, deafness, language difficulties, or any other disability which may prevent
the participant.

Excluded Medications:

- Experimental drugs.

- Coumadin, heparin, or any other anticoagulant.

- Insulin or other hypoglycemic agents.

- Supplements containing more than 50 µg selenium/day (US RDA) in the 12 weeks prior to
screening.