Sleep Quality and Amyloid-Beta Kinetics

The purpose of the study is to investigate whether or not increasing sleep efficiency will
change the concentration of amyloid-beta in cerebrospinal fluid (CSF).

Inclusion Criteria:

- Age 45-65 years

- Any sex

- Any race/ethnicity

- Mini-Mental Status Examination score (MMSE) >=27

- Sleep efficiency measured by actigraphy to determine good sleeper or poor sleeper

Exclusion Criteria:

- Cognitive impairment as determined by history of MMSE < 27

- Inability to speak or understand English

- BMI >35

- Any sleep disorders other than insomnia

- history of sleep-disordered breathing

- STOP-Bang score > 3

- History or reported symptoms suggestive of restless legs syndrome, narcolepsy or other
sleep disorders

- Sleep schedule outside the range of bedtime 8PM-12AM and wake time 4AM-8AM

- Contraindication to lumbar catheter (anticoagulants; bleeding disorder; allergy to
lidocaine or disinfectant; prior central nervous system or lower back surgery)

- Cardiovascular disease requiring medication except for controlled hypertension (PI
discretion)

- Stroke

- Hepatic or renal impairment

- Pulmonary disease (PI discretion)

- Type 1 diabetes

- HIV or AIDS

- Neurologic or psychiatric disorder requiring medication (PI discretion)

- Alcohol or tobacco use (PI discretion)

- Use of sedating medications

- Inability to get out of bed independently

- Abnormal movement of the non-dominant arm (would affect actigraphy data in
unpredictable ways)

- Abnormal physical examination

- Current pregnancy