Investigation of the Effects of Electronic Cigarettes on Vascular Health

Inclusion Criteria:

- Male or female between 18 and 50 years of age.

- Good general health with no history of diabetes, coronary artery disease, peripheral
arterial disease, chronic disease or hypertension.

- Ability to sign an informed consent.

Tobacco product users will have additional inclusion criteria:

- For TC smokers, daily TC smoking in the past 6 months, at least 10 cigarettes per day,
with no EC exposure in the past 6 months

- For EC users, daily EC use in the past 6 months, at least 10 sessions per day, with no
TC exposure in the past 6 month

- For non-smokers, no significant lifetime exposure to any nicotine-containing product,
where significant exposure is defined as daily use of any nicotine-containing product
for more than one week or once monthly use for more than 6 months.

Exclusion Criteria:

- History of renal disease, hypertension, diabetes, congestive heart failure or
emphysema

- Use of ACE inhibitors, Angiotensin II receptor blockers, diuretics, aldosterone, renin
blockers, aspirin, statins, sildenafil (or other PDE5 inhibitors) and NSAIDs.

- History of substance abuse

- Currently using nicotine replacement or other tobacco cessation products or
intentionally abstaining from nicotine-containing products

- IV contrast exposure in the past 1 month

- Inability to place an IV catheter or draw blood for any reason

- Pregnant women or breastfeeding

- Potential subjects will be asked if they are pregnant. Verbal confirmation of
pregnancy will be sufficient.

- Fever of >101°F or BP >180/95

- BMI ≥30