Studying Biomarkers in Tumor Tissue and Blood Samples From Patients With Small Cell Lung Cancer Registered on CALGB-140202

OBJECTIVES:

Primary

- To identify the major genetic alterations in human small cell lung cancer (SCLC) using
next-generation sequencing and focusing on mutations in coding sequence.

Secondary

- To follow up with in-depth assessment of candidate oncogenes and tumor suppressor genes
using gene expression analysis, cell culture systems, and murine models of SCLC.

OUTLINE: DNA isolated from archived tumor tissue and blood samples are analyzed for genetic
mutations and gene expression profiling using Illumina exome sequencing. Results are
compared with transcriptome of tumors (or cell lines) with and without the specific
mutation. Cell culture studies overexpressing or inhibiting the genes of interest are also
performed in a mouse model to identified potential drivers of small cell lung cancer.

- Patients must have been registered to CALGB-140202 trial in which tumor material
(frozen tissue and paired blood specimens) is available

- Institutional Review Board (IRB) review and approval at the institution where the
laboratory work will be performed is required