Studying Genes in Tissue Samples From Younger and Adolescent Patients With Soft Tissue Sarcomas

Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective:
Retrospective Biospecimen Retention: Samples With DNA Biospecimen Description: Tissue Study
Population Description: Existing NRSTS samples from the COG D9902/ARST0332 studies Sampling
Method: Non-Probability Sample

OBJECTIVES:

I. To determine the frequency with which actionable mutations are found in archived
non-rhabdomyosarcoma soft tissue sarcoma (NRSTS) tumor specimens using mass spectrometry
(MS) analysis of tumor-derived deoxyribonucleic acid (DNA).

OUTLINE:

Archived DNA tissue samples are analyzed for frequency of genetic mutations, including
single nucleotide polymorphisms (SNPs), single nucleotide variants (SNVs), and small
deletions and/or insertions, by polymerase chain reaction (PCR) and mass spectometry
(Sequenom MassARRAY). Results are then analyzed to determine whether specific mutations
correlate with patient or disease features such as tumor stage, histological grade, or
outcome.

Inclusion Criteria:

- Archived non-rhabdomyosarcoma soft tissue sarcoma (NRSTS) tumor-derived DNA

- Synovial sarcoma, malignant peripheral nerve sheath tumor (MPNST), soft tissue
sarcoma not otherwise specified (NOS), or other less common pediatric NRSTS

- Formalin-fixed, paraffin-embedded (FFPE) tissue from patients enrolled on:

- COG-D9902 Soft Tissue Sarcoma (STS) Biology and Banking Protocol

- COG-ARST0332 A Risk-Based Treatment for Pediatric NRSTS Study

- See Disease Characteristics