Study of Tumor Samples From Patients With Ewing Sarcoma

Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective:
Prospective Biospecimen Retention: Samples With DNA Biospecimen Description: Tissue Study
Population Description: Samples collected from AEWS0031 and patients registered on AEWS08B1
Sampling Method: Non-Probability Sample

PRIMARY OBJECTIVES:

I. Determine if mutation of p53, and/or deletion of the p16 locus, have prognostic value in
patients with Ewing sarcoma.

SECONDARY OBJECTIVES:

I. Estimate the incidence of p53 mutation in Ewing sarcoma samples collected from COG
studies.

II. Estimate the incidence of p16 deletions in Ewing sarcoma samples collected from COG
studies.

III. Prepare and archive amplified genomic DNA from Ewing sarcoma samples collected from COG
studies for future biologic analysis.

OUTLINE: This is a multicenter study.

Previously archived tumor samples are analyzed for p53 mutations and p16 deletion by
immunohistochemistry, FISH, PCR, and DNA sequencing.

Inclusion Criteria:

- Diagnosis of Ewing sarcoma

- Banked specimens from patients enrolled on AEWS0031