Methoxyamine and Temozolomide in Treating Patients With Recurrent Glioblastoma

PRIMARY OBJECTIVES:

I. To estimate the efficacy of TRC102 (methoxyamine) and temozolomide, as measured by
response rate, in bevacizumab naïve glioblastoma. (Arm I) II. To estimate the efficacy of
TRC102 and temozolomide, as measured by response rate, in bevacizumab refractory
glioblastoma. (Arm II)

SECONDARY OBJECTIVES:

I. Evaluate the toxicities of oral TRC102 and temozolomide in this patient population.

II. Estimate the efficacy of TRC102 and temozolomide, as measured by progression-free
survival, progression-free survival at 6 months and overall survival, in bevacizumab naïve
glioblastoma.

III. Estimate the efficacy of TRC102 and temozolomide, as measured by progression-free
survival in bevacizumab refractory glioblastoma.

TERTIARY OBJECTIVES:

I. Assess the tissue correlates of N-methylpurine-deoxyribonucleic acid (DNA) glycosylase
(MPG), topoisomerase II-alpha (topo II a), and O-6-methylguanine-DNA methyltransferase (MGMT)
status, with response, progression-free survival (PFS), and overall survival.

OUTLINE:

Patients receive methoxyamine orally (PO) once daily (QD) and temozolomide PO QD on days 1-5.
Courses repeat every 28 days in the absence of disease progression or unacceptable toxicity.

After completion of study treatment, patients are followed up for 30 days, every 2 months for
2 years, and then every 6 months thereafter.

Inclusion Criteria:

- Patients must have histologically confirmed glioblastoma that is progressive or
recurrent following radiation therapy and temozolomide

- Tumor MGMT methylation status must be available; results of routinely used methods for
MGMT methylation testing (e.g. methylation-specific polymerase chain reaction [MSPCR ]
or quantitative polymerase chain reaction [PCR]) are acceptable

- Arm 1 patients must have measurable (defined by at least 1 cm x 1 cm)
contrast-enhancing disease by magnetic resonance imaging (MRI) imaging within 21 days
of starting treatment

- Patients must be able to undergo MRI of the brain with gadolinium; patients must be
maintained on a stable or decreasing dose of corticosteroid regimen (no increase for 5
days) prior to this baseline MRI

- Arm 1 patients must be in first recurrence of glioblastoma following radiation therapy
and temozolomide

- Arm 2 patients may have an unlimited number of prior therapy regimens but may not have
received prior antiangiogenesis therapy except for bevacizumab (patients may not have
received aflibercept, ramucirumab, cediranib, cabozantinib, or XL184)

- Arm 1 patients must have not received bevacizumab previously

- Arm 2 patients must have progressed/recurred on bevacizumab as the most recent
regimen; patients on arm 2 should continue on bevacizumab as clinically necessary to
control brain edema

- Patients must have a tumor tissue form indicating availability of archived tissue from
initial resection at diagnosis of glioblastoma completed and signed by a pathologist;
availability of tissue is not a requirement for study participation

- Patients must have recovered from severe toxicity of prior therapy; the following
intervals from previous treatments are required to be eligible:

- 12 weeks from the completion of radiation

- 6 weeks from a nitrosourea chemotherapy

- 3 weeks from a non-nitrosourea chemotherapy

- 4 weeks from any investigational (not Food and Drug Administration
[FDA]-approved) agents

- 2 weeks from administration of a non-cytotoxic, FDA-approved agent (e.g.,
erlotinib, hydroxychloroquine, etc.)

- Patients must have a Karnofsky performance status >= 60% (i.e. the patient must be
able to care for himself/herself with occasional help from others)

- Absolute neutrophil count >= 1,500/mcL

- Platelets >= 100,000/mcL

- Hemoglobin >= 9 g/dL

- Total bilirubin =< institutional upper limit of normal

- Aspartate aminotransferase (AST) (serum glutamic oxaloacetic transaminase
[SGOT])/alanine aminotransferase (ALT) (serum glutamate pyruvate transaminase [SGPT])
=< 4 x institutional upper limit of normal

- Creatinine =< institutional upper limit of normal OR creatinine clearance >= 60
ml/min/1.73 m^2 for patients with creatinine levels above institutional normal

- Activated partial thromboplastin time (APTT) or partial thromboplastin time (PTT) =<
1.5 x institutional upper limit of normal

- Patients must be able to provide written informed consent

- Women of childbearing potential must have a negative serum pregnancy test prior to
study start; women of childbearing potential and men must agree to use adequate
contraception (hormonal or barrier method of birth control; abstinence) prior to study
entry, for the duration of study participation, and through 30 days after the last
dose of study drug; should a woman become pregnant or suspect she is pregnant while
participating in this study, she should inform her treating physician immediately; men
treated or enrolled on this protocol must also agree to use adequate contraception
prior to the study, for the duration of study participation, and through 30 days after
the last dose of study drug

- Patients must have no concurrent malignancy except curatively treated basal or
squamous cell carcinoma of the skin or carcinoma in situ of the cervix, breast, or
bladder; patients with prior malignancies must be disease-free for >= five years

- Patients must be able to swallow capsules

Exclusion Criteria:

- Patients receiving any other investigational agents are ineligible

- Patient must not have known sensitivity to TRC102 or any formulation excipients

- Patients on enzyme-inducing anti-epileptic drugs (EIAED) are not eligible for
treatment on this protocol; patients may be on non-enzyme inducing anti-epileptic
drugs or not be taking any anti-epileptic drugs; patients previously treated with
EIAED may be enrolled if they have been off the EIAED for 10 days or more prior to the
first dose of TRC102

- Patients must not be on any anticoagulation

- Patient must not have prior gastrointestinal (GI) surgery or GI disease that might
interfere with the absorption of TRC102

- Patients who have not recovered to < Common Terminology Criteria for Adverse Events
(CTCAE) grade 2 toxicities related to prior therapy are ineligible

- Patients must not have active brain metastases from a systemic solid tumor

- Patients with uncontrolled intercurrent illness including, but not limited to, ongoing
or active infection, symptomatic congestive heart failure, clinically significant
cardiac disease, unstable angina pectoris, cardiac arrhythmia, or psychiatric
illness/social situations that would limit compliance with study requirements, are
ineligible

- Pregnant women are excluded from this study; breastfeeding should be discontinued if
the mother is treated with TRC102

- Human immunodeficiency virus (HIV)-positive patients on combination antiretroviral
therapy are ineligible