Does Meal Timing Affect Energy Expenditure

10 overweight adult men and women will eat according to two different eating schedules:
grazing and time-restricting feeding ("early eating"). While on each eating schedule,
metabolism (calories burned) will be measured during a 24-hour stay in a respiratory chamber.
Glucose levels—as well as key diurnal rhythms such in heart rate—will also be measured
continuously. This study requires two 1-week periods of participation.

Inclusion Criteria:

- Are 20-45 years of age

- Have a weight between 150 and 220 lbs

- Have a body mass index between 25 and 35 kg/m2 inclusive (a number calculated from
your height and weight)

- If you are a female, have a regular menstrual cycle that is between 25 and 35 days
long

- If you are female, had your period within the last 35 days

- Regularly go to sleep between 9:30 pm and 12:00 am

- Regularly eat dinner 9 or more hours after eating breakfast

- Are willing to have about 2/3 cup of your blood stored for future research related to
this study

Exclusion Criteria:

- Have diabetes or are on anti-diabetes medication

- Suffer from significant cardiovascular, renal (kidney), cardiac (heart), liver, lung
or nervous system disease

- Have stomach or intestinal problems

- Regularly use medications such as steroids, beta blockers, adrenergic-stimulating
agents, and laxatives

- Take any medications or supplements known to affect sleep, circadian rhythms, or
metabolism (with the exception that certain forms of birth control are allowed)

- Have abnormal lab work that is clinically significant in the opinion of the study
physician

- Are pregnant or lactating

- Take the Depo Provera shot, or use an Interuterine Device (IUD) or hormone patch for
birth control

- If you are on hormonal birth control, have been on a stable dose for less than 3
months

- Have smoked or used nicotine/tobacco products within the last 3 months

- Regularly do competitive sport training

- Perform overnight shift work an average of one or more times per week

- Have an irregular sleep schedule

- Have an irregular eating schedule

- Regularly drink 3 or more drinks of alcohol per day

- Are not able or are unwilling to eat only the food and drink served to you by
Pennington Biomedical, while in the study

- Are not able or are unwilling to refrain from strenuous exercise while participating
in the study

- Are not able to spend 2 separate full days (for about 25 hours each) at Pennington
Biomedical

- Are not able to maintain a consistent sleep schedule while participating in the study

- Are not able to maintain a consistent eating schedule while participating in the study

- Are not able to stop drinking coffee and other caffeine-containing drinks on the day
of and day prior to testing