The Effects of Minocycline in Humans

We are proposing a randomized, double-blind, crossover study. A total of 40 male and or
female subject completers will have two 4-day treatment periods, in which they will be
randomized to minocycline (200 mg/day) or placebo. During the first 3 days of each treatment
period, subjects will have daily clinic visits for medication administration and monitoring
of adverse events. On Day 4, subjects will have an experimental session in which measures of
mood and cognitive performance will be obtained. Following a washout period, ranging from 5
to 15 days, subjects will be crossed over to the alternative treatment.

This study began July 2013; currently 7 subjects were randomized with 5 completers and two
drop outs. This study is active with recruitment continuing.

Inclusion Criteria:

- Male and females, between the ages of 21 and 50 who fulfill criteria for past cocaine
dependence according to DSM-IV criteria;

- No cocaine use for the past 30 days;

- No other current dependence or abuse of other drugs of abuse or alcohol (except
cocaine and tobacco);

- No current medical problems and normal ECG;

- For women, not pregnant as determined by pregnancy screening nor breast feeding, and
using acceptable birth control methods.

Exclusion Criteria:

- current major psychiatric illnesses including mood, psychotic, or anxiety disorders;

- history of major medical illnesses; including liver diseases, heart disease, or other
medical conditions that the physician investigator deems contraindicated for the
subject to be in the study

- Current use of over-the-counter or prescription psychoactive drugs (antidepressant,
anxiolytics, antipsychotics, mood stabilizers, psychostimulants) or drugs that would
be expected to have major interactions with drugs to be tested, e.g., benzodiazepines,
codeine, percocet, and other opiate drugs that will interact with methadone.

- Liver function tests (ALT or AST) greater than 3 times normal.

- Allergy to minocycline or other tetracyclines.