Investigation of the Gut Microbiota in Regulating Nutrient Absorption in Humans



Status:Completed
Conditions:Obesity Weight Loss
Therapuetic Areas:Endocrinology
Healthy:No
Age Range:18 - 45
Updated:4/6/2019
Start Date:January 14, 2014
End Date:March 29, 2019

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The prevalence of obesity has risen to epidemic proportions in the world, resulting from both
excessive energy intake and low levels of energy expenditure. The effect of nutrient
absorption on energy balance, that is, the relative amount of nutrients consumed vs. the
amount excreted in stool, has been reported only in small studies in which energy waste in
feces and urine between lean and obese individuals was not found to be different. New studies
have shown that bacteria in the gut may play an important role in calorie absorption. We have
recently shown that leaner individuals absorbed more calories when overfed compared to when
they were given a diet with just enough calories to maintain their own weight. Our studies
have also found that overfeeding also changes the kinds of bacteria found in the gut. In lean
individuals, these changes in gut bacterial communities with overfeeding were associated with
changes in how many calories were absorbed. Our results are similar to those seen in other
studies in animals and humans that suggest a role for gut bacteria in weight gain and
obesity. To try to better understand the role of gut bacteria in absorbing food, we propose
to investigate 1) whether energy loss (as measured in stool and urine) changes following
over- and underfeeding relative to body size and 2) whether changes in the gut bacteria,
induced by an antibiotic medication, affect nutrient absorption and glucose tolerance. We
plan to study 24 healthy non-smoking volunteers age 18 45 years old, not taking any
medications (including medications for weight loss, antibiotics or probiotics) for the
examination. All participants will be admitted to the Clinical Research Unit for 31 days.
During their stay, subjects will be fed a weight maintaining diet for 3 days, followed by two
experimental diets (150% and 50% of weight maintaining calories) in a random order. After
this, volunteers will be randomly assigned to one of two groups: group 1 will take oral
antibiotic medication; group 2 will receive pills that look the same but will not contain any
active medication (placebos). Feces (stool) will be collected throughout the study.
Additionally, twenty four-hour urine collections will take place each day of the experimental
diet period and when stool is collected on the antibiotics. The energy content of these waste
products as well as that of the diet (using duplicate plate analysis) will be measured by
bomb calorimetry. Bacterial components in feces will be extracted by repeated fractional
centrifugation to obtain bacterial mass and by using 16S rDNA-based oligonucleotide probes to
obtain data on gut bacteria. Primary results will examine how many calories remain in stool
during relative over- and underfeeding and whether changes in gut bacteria, induced by an
antibiotic medication, affect nutrient absorption and glucose tolerance.

The prevalence of obesity has risen to epidemic proportions in the world, resulting from both
excessive energy intake and low levels of energy expenditure. The effect of nutrient
absorption on energy balance, that is, the relative amount of nutrients consumed vs. the
amount excreted in stool, has been reported only in small studies in which energy waste in
feces and urine between lean and obese individuals was not found to be different. New studies
have shown that bacteria in the gut may play an important role in calorie absorption. We have
recently shown that leaner individuals absorbed more calories when overfed compared to when
they were given a diet with just enough calories to maintain their own weight. Our studies
have also found that overfeeding also changes the kinds of bacteria found in the gut. In lean
individuals, these changes in gut bacterial communities with overfeeding were associated with
changes in how many calories were absorbed. Our results are similar to those seen in other
studies in animals and humans that suggest a role for gut bacteria in weight gain and
obesity. To try to better understand the role of gut bacteria in absorbing food, we propose
to investigate 1) whether energy loss (as measured in stool and urine) changes following
over- and underfeeding relative to body size and 2) whether changes in the gut bacteria,
induced by an antibiotic medication, affect nutrient absorption and glucose tolerance. We
plan to study 24 healthy non-smoking volunteers age 18 45 years old, not taking any
medications (including medications for weight loss, antibiotics or probiotics) for the
examination. All participants will be admitted to the Clinical Research Unit for 31 days.
During their stay, subjects will be fed a weight maintaining diet for 3 days, followed by two
experimental diets (150% and 50% of weight maintaining calories) in a random order. After
this, volunteers will be randomly assigned to one of two groups: group 1 will take oral
antibiotic medication; group 2 will receive pills that look the same but will not contain any
active medication (placebos). Feces (stool) will be collected throughout the study.
Additionally, twenty four-hour urine collections will take place each day of the experimental
diet period and when stool is collected on the antibiotics. The energy content of these waste
products as well as that of the diet (using duplicate plate analysis) will be measured by
bomb calorimetry. Bacterial components in feces will be extracted by repeated fractional
centrifugation to obtain bacterial mass and by using 16S rDNA-based oligonucleotide probes to
obtain data on gut bacteria. Primary results will examine how many calories remain in stool
during relative over- and underfeeding and whether changes in gut bacteria, induced by an
antibiotic medication, affect nutrient absorption and glucose tolerance.

- INCLUSION CRITERIA:

Free of acute and chronic diseases (especially GI disorders) as determined by medical
history, physical examination and laboratory tests.

Individuals may be taking laxative drugs but they must be discontinued 3 or more weeks
before admission.

Age 18-45 y (in order to minimize the affect of aging on nutrient absorption).

EXCLUSION CRITERIA:

Because it is unclear how chronic illnesses or substance abuse could affect nutrient
absorption we will exclude volunteers with chronic diseases or current substance abuse.
This is especially important because the limited number of study subjects in this study
will make it hard to control for these confounders. We will therefore exclude subjects with
a history or clinical manifestation of:

- Current smoking

- Type 2 diabetes (according to the World Health Organization diagnostic criteria)

- Endocrine disorders, such as Cushing s disease, pituitary disorders, and hypo- and
hyperthyroidism

- HIV infection (self-report), due to effects on weight and body composition of HIV and
medications used to treat HIV

- Active tuberculosis (self-report)

- Asthma on active daily treatment with medications

- Pulmonary disorders including physician diagnosed chronic obstructive pulmonary
diseases and obstructive sleep apnea syndrome

- Cardiovascular diseases, including coronary heart disease, heart failure, arrhythmias,
and peripheral artery disease

- Hypertension (according to the World Health Organization diagnostic criteria), treated
or uncontrolled

- Gastrointestinal disease, including inflammatory bowel diseases (e.g. Crohn s disease
and ulcerative colitis), malabsorption syndromes (e.g. celiac disease), gastric ulcer
(active) and irritable bowel syndrome.

- Lactose intolerance

- Anemia (defined as hemoglobin < 11 mg/dl), leucopenia (defined as white blood cell
count < 4,000/microL) or thrombocytopenia (defined as platelet count < 150,000/microL)

- Liver disease, including non-alcoholic fatty liver disease or current elevated liver
enzymes over 1.5 times the normal range for AST, ALT or GGT or a history and physical
exam that indicates a potential liver disease as describe by Giannini et al

- Evidence of chronic renal disease as defined by estimated glomerular filtration rate
of < 60 ml/min or evidence of overt proteinuria on urine dipstick.

- Central nervous system disease, including previous history of cerebrovascular
accidents, dementia, and neurodegenerative disorders

- Cancer requiring treatment in the past five years, except for non-melanoma skin
cancers or cancers that have clearly been cured or in the opinion of the investigator
carry an excellent prognosis

- Behavioral or psychiatric conditions that would be incompatible with a safe and
successful participation in the study (such as major depression, schizophrenia and
presence of psychotic symptoms)

- Eating disorders such as anorexia nervosa, bulimia or binge eating syndrome

- Taking weight loss drugs

- Weight change of more than 5% of total body weight in the 3 months before admission

- Use of any antibiotic or probiotic agents within 6 months prior to minimize the
potential effects of these substances on the gut microbiota.

- Use of antacids (Proton pump inhibitors, H2 antagonists or aluminum/magnesium
hydroxide) 3 months prior to the study assessed by self-report because a modified
gastric pH might affect the gut microbiota as well

- Evidence of alcohol and/or drug abuse (more than 3 drinks per day and use of drugs,
such as amphetamines, cocaine, heroin, or marijuana)

The following exclusion criteria are necessary because of the substances given or tests
performed during the study

- Known allergies to vancomycin

- Known allergies to heparin or a history of heparin-induced thrombocytopenia

- Personal history or evidence of a bleeding disorder

All individuals will be fully informed of the aim, nature, and risks of the study prior to
giving written informed consent. The study s informed consent will be obtained by a
principal or associate investigator, research physician or physician assistant working in
the clinical research unit.
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Phoenix, Arizona 85014
Phone: 602-200-5217
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Phoenix, AZ
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