Brain Mechanisms of Emotion and Motivation: A Mind-Body Study

The purpose of this research study is to investigate how the brain processes emotions, and
the way these processes affect behavior. Specifically, we hypothesize that individual
differences in reward responsiveness will correlate with differential activation in
mesolimbic regions of the brain and predict future wellbeing in follow-up interviews.
Participating in this study involves up to two sessions and up to 7.5 hours. In the present
observational study, each participant will be exposed to the same experimental procedure.

Visit 1 will take about 4.5 hours, from 12pm to 4:30pm. The visit will take place at the
Center for Depression, Anxiety and Stress at McLean. During this visit, a clinical
interviewer will conduct a diagnostic SCID interview to ensure that participants qualify to
take part in this research study. Participants will then complete several tasks. These tasks
include several different "game-like" situations and arithmetic riddles, all of which are
simple but require participants to work hard and remain alert for an extended period of time.
The goal during these games is to earn as much money as you can or to avoid losing money.
Participants will do so by responding (pressing a keyboard button) quickly and accurately.
These tasks are intended as assessment tools, and are not a form of therapy or intervention.
In addition, we will test participants' ability to keep their hand in cold water.
Participants will also be asked to provide blood (via a saline IV) and saliva samples at
different times during this session, and we will also conduct urine drug and pregnancy tests.
The IV will be in place for a total of around 3 hours, and is used to reduce discomfort from
multiple blood draws.

Visit 2 will take place at the McLean Imaging Center at McLean Hospital. During the first
part of the session, the fMRI scanner will take structural pictures of brain anatomy. Next,
additional images will be obtained while participants complete several different "game-like"
tasks, similar to the tasks performed in visit 1. Again, these tasks are intended as
assessment tools, and are not a form of therapy or intervention. The whole fMRI session will
last up to about 2.5 hours; participants will spend about 1.5 hours in the fMRI machine, and
about a half-an-hour completing questionnaires and providing saliva samples. Prior to the
fMRI scan, another urine sample will be obtained to check for drugs which may interfere with
the experiment.

After the second study session is complete, participants will be re-contacted through the
internet every four weeks for a total of six months, to complete some questionnaires. At the
end of six months, participants will be asked to return to McLean to complete an in-person
interview. This follow-up period is purely observational and not intended to assess health
outcomes, as there is no intervention in this study.

Inclusion Criteria:

- Absence of medical, neurological, and psychiatric illness (including alcohol and
substance abuse), as assessed by subject history and a structured clinical interview
(SCID-I/NP)

- Written informed consent

- Both genders and all ethnic origins, age between 18 and 45

- Right-handed

- Absence of any medications for at least 3 weeks

- Absence of pregnancy as determined by completion of a negative urine pregnancy test
prior to the MRI scan

- Fluent English speaker

- Absence of current drug use (cocaine, cannabinoids, opiates, amphetamines,
methamphetamines, phencyclidine, MDMA, benzodiazepines, methadone, oxycodone,
tricyclic antidepressants, and barbiturates) as assessed by a urine drug test.

Exclusion Criteria:

- Pregnant or currently breast-feeding women or any woman of childbearing potential who
is seeking to become pregnant or suspects that she may be pregnant.

- History or current serious or unstable medical illness, including cardiovascular,
hepatic, renal, respiratory, endocrine, neurologic or hematologic disease

- History of seizure disorder

- Failure to meet standard fMRI safety requirements

- History of head injury

- History or current diagnosis of any DSM-IV psychiatric illnesses; Presence of a DSM-IV
psychiatric illness within a first-degree relative

- History of cocaine or stimulant use (e.g., amphetamine, cocaine, methamphetamine)

- History of use of dopaminergic drugs (including methylphenidate)

- History or current diagnosis of dementia

- Patients with mood congruent or mood incongruent psychotic features

- History or current use of any psychiatric medication

- Current use of any drug or herbal supplement with well-characterized psychotropic
effects (e.g. St. John's wort)

- Clinical or laboratory evidence of hypothyroidism

- Diabetes with poor glucose control

- Currently taking medication that affects blood flow, e.g. certain blood pressure
medications

- Evidence of significant inconsistencies in self-report.