Biomarker Expression in Tissue Samples From Patients With Bone Sarcomas

STUDY SUBTYPE: Ancillary/Correlative

OBSERVATIONAL STUDY MODEL: Case-only

TIME PERSPECTIVE: Retrospective

BIOSPECIMEN RETENTION: Samples without DNA

BIOSPECIMEN DESCRIPTION: Tissue samples obtained from Cooperative Human Tissue Network
(CHTN)

STUDY POPULATION DESCRIPTION: Patients enrolled on P9754 and AOST0121 clinical trials

SAMPLING METHOD: Non-probability sample

PRIMARY OBJECTIVES:

I. To determine if there is differential expression of tumor factor (TF) among patients with
osteosarcoma.

II. To determine if there is an association between overall survival and disease free
survival and the extent of TF expression.

III. To determine if TF expression correlates with presence of gross metastatic disease at
presentation, and development of subsequent metastases.

OUTLINE:

Tissue samples are analyzed for tumor factor expression levels by immunohistochemistry.

Inclusion Criteria:

- Tissue samples from patients with osteosarcomas obtained from the CHTN:

- P9754 clinical trial for patients with non-metastatic osteosarcoma

- AOST0121 clinical trial for patients with metastatic or recurrent osteosarcoma