Evaluating Immune Response to Seasonal FluMist in Healthy Adults

This is a VA-based study to evaluate serum and nasal wash antibody responses to seasonal LAIV
of healthy Veterans and VA employees between the ages of 20 and 49 years old.

Specific Aims

1. The primary aim of the study is to evaluate whether people with high mucosal IgA
antibodies against flu have a lower immune response to the seasonal LAIV.

2. The secondary objective is to determine whether the nasal wash viral neutralization data
predicts LAIV immunization failures.

Inclusion Criteria:

- Medically eligible healthy men and women between the ages of 20 to 49 years old.

Exclusion Criteria:

- A medical condition that places them at high risk for complications from influenza,
including those with chronic heart or lung disease, such as asthma or reactive airway
disease; people with medical conditions such as diabetes or kidney failure; or people
with illnesses that weaken the immune system, or who take medications that can weaken
the immune system.

- A history of Guillain-Barré Syndrome that occurred after receiving influenza vaccine

- Currently pregnant

- Have a severe allergy to chicken eggs or who are allergic to any of the nasal spray
vaccine components

- Have had flu within the current flu season.

- Have had a flu vaccine within the current flu season.

- Have close contact with someone whose immune system is so weak they require care in a
protected environment (such as bone marrow transplant unit).