Interstitial Cystitis: Elucidation of the Psychophysiologic and Autonomic Characteristics (ICEPAC) Study

The investigators primary hypothesis is that IC/PBS is a member of a larger family of
disorders sharing aberrant central autonomic and sensory response to stress, pain or threat.
These disorders appear to share a common vulnerability that seems to be conferred during
development, and symptoms of these disorders usually are first manifested in response to an
environmental trigger. This proposal aims to compare the neural, psychological, and
endocrine phenotypes that characterize patients with IC/PBS with those of patients suffering
myofascial pelvic pain (MPP) syndrome, an chronic pelvic pain distinct from IC/PBS,
age-matched, healthy controls, and first degree relatives. These studies are designed to
identify which levels of the neuraxis are impaired, both in the basal state, and in response
to a well-characterized psychosocial stressor.

Aim 1: To differentiate the specific baseline neurophysiological abnormalities that occur in
IC/PBS from those present in patients with MPP and healthy subjects, specifically:

1a: Bladder and pelvic floor afferent and efferent urogynecological function: (1) voiding
diaries (efferent) modified to include void-state related numeric rating scales for pain
(afferent); Uroflow measurements (efferent), and a double-blind placebo-controlled
evaluation of the diagnostic lidocaine instillation test (afferent) with impact on voiding
function (efferent); (2) semi-quantitative evaluation of pelvic floor function and
identification of myofascial trigger points (efferent), including inter-observer validation
of a standardized semi-quantitative examination (afferent); (3) quantitative Q-tip test for
vulvodynia (afferent) (4) evaluation of dysmenorrhea (afferent) and menstrual function
(efferent).

1b: somatic afferent and autonomic efferent neural function, specifically: (1) global screen
for autonomic and neurological abnormalities through the established Small Fiber Score
Instrument (SFIBS) questionnaire and structured neurological examination (afferent and
efferent); (2) specific evaluation of sacral and lumbar nerve root function through a
focused neurological examination (afferent and efferent); (3) parasympathetic cardiac
function through the cardiac response to deep breathing (efferent); (4) sympathetic cardiac
and vasomotor functions through the cardiovascular responses to the Valsalva maneuver and to
an upright tilt table test (efferent); (5) sudomotor sympathetic function through the
quantitative sudomotor axon reflex test (QSART) that evaluates post-ganglionic function
(specifically abnormal in autonomic neuropathies) and through a thermoregulatory sweat test
(efferent).

1. c: gastrointestinal afferent and efferent function, specifically upper bowel motility
with established methods: (1) early satiety & gastric compliance by water load test
(afferent). (2) gastric electrical activity through electrogastrography (efferent).

Aim 2: To determine the specific developmental, psychiatric, pain, autonomic, and
stress response characteristics common to IC/PBS and their family members, that differ
from MPP and healthy subjects through:

2. a: Stress and trauma history in early childhood and adulthood.

2b: Psychiatric screening and psychometric quantitation of psychological symptoms, pain and
function.

2c: Quantitation of associated co-morbid autonomic disorders through the ODYSA
questionnaire.

2d: Salivary cortisol levels immediately prior to autonomic testing (anticipatory stress)
and after a period of relaxation once the test is finished, in conjunction with a stress
self-assessment inventory.

2e: Performance of the Trier test on a subset of patients and controls, with measurement of
autonomic cardiovascular parameters, body temperature, catecholamine concentrations
(norepinephrine, epinephrine, dopamine) and endocrine parameters: ACTH and adrenocortical
hormones.

General exclusion criteria for all IC/PBS and MPP patients, their siblings, and normal
subjects will include presence of:

1. Currently attempting to become pregnant, pregnant (pregnancy test will be required)
or breast feeding

2. Hematuria (? this can occur in IC) or infection on urinalysis

3. Recurrent urinary tract infections (> 3 culture documented episodes within the
previous 12 months)

4. Pelvic or bladder neoplasm or infection

5. Inflammatory arthritis, connective tissue or auto-immune disorder

6. Evidence of unstable medical disorder, such as kidney (rising creatinine, or
end-stage renal failure) or liver impairment (rising AST or ALT, or end-stage with
coagulopathy), poorly controlled significant cardiovascular (CHF), respiratory,
endocrine (diabetes - A1c > 9 - or untreated thyroid dysfunction) or uncontrolled
psychiatric illness (such as untreated depression, psychosis, etc.).

7. Neuropathy, central nervous system disorder (e.g., Parkinson's Disease, Alzheimer's,
MS, stroke, etc)

8. Treatment with a drug or medical device within the previous 30 days that has not
received regulatory approval

9. Use of hormones (except insulin, thyroid replacement or oral contraceptives, which
will be carefully documented)

10. Regular use of opiods

11. Allergy to lidocaine

12. Inability to stop use of autonomically active or pro-kinetic (gastrointestinal
motility modifying) agents for a minimum of 5 half-lives prior to testing

13. Current substance abuse or > 10 alcoholic beverages per week

14. Any major surgical intervention with general anesthesia in the last 90 days

15. Any on-going or pending medical, health or disability related litigation, or current
pursuit of disability

16. Any condition that in the judgment of the investigator and the internal advisory
panel would interfere with the patient's ability to provide informed consent, comply
with study instructions, place the patient at increased risk, or which would clearly
confound the interpretation of the study results (specific reason will be documented)
17.Investigators, study staff and their immediate families 18.Inability to speak
English

19) Previously completed or withdrawn from this study

Normal controls and siblings: Aged-matched healthy female subjects will be recruited by
nomination by patients and advertisement. A history and physical will be obtained by a
physician, and controls will be required to have no history, symptoms or signs of
(exclusion criteria):

1. FM or CFS (unexplained fatigue for a period of 6 months or more)

2. IC/PBS, MPP or chronic pelvic discomfort or chronic pain disorder of any type.

3. One of the other ODYSA dysautonomias

IC/PBS Patients - The diagnosis of IC/PBS will be made using most current NIDDK criteria.
Exclusion criteria:

1. Intravesical therapy or bladder hydrodistention within the previous 90 days

2. Initiation of pentosan polysulfate sodium (Elmiron) within the previous 90 days

3. Previous augmentation cystoplasty, cystectomy or cytolysis, neurectomy (i.e.,
hypogastric nerve plexus ablation) or implanted neural stimulator which is
functionally "on", in active use, or unable to be turned functionally off throughout
the study period.

MPP Patients - Inclusion Criteria

1. CPP > 3 months duration, with pain ranked > 3/10 by oral analog scale

2. Presence of 1 or more palpable trigger points on transvaginal and/or transrectal
examination of the pelvic floor, which reproduces the pain for which they are seeking
medical care.

Exclusion criteria:

1. Pelvic surgery within the last 12 months

2. Pelvic injection with the last 90 days

3. Presence of IC/PBS by the current NIDDK criteria