Protocol to Obtain Bone Marrow for Research

Therapuetic Areas:Hematology
Age Range:17 - Any
Start Date:June 2005
End Date:December 2018

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Potential donors who are undergoing bone marrow biopsy or bone marrow donation as part of
clinical care will be asked by the principal investigator (PI) if they might be interested in
donating marrow for this research project. If they are interested, the PI will meet with the
prospective donor to review the information contained in the informed consent document and to
answer any questions the potential subject may have. Bone marrow aspirates of approximately
5cc will be obtained from the posterior iliac crest of subjects using standard techniques.

The purpose of this study is to determine the mechanisms that regulate human globin gene
expression by analyzing the function of cell signaling pathways and the expression of other
related genes and their protein products in the bone marrow cells of people without blood
diseases. This includes the effects of drugs and other in vitro manipulations of the cells
once they are removed from donors. These assays will include, but are not limited to,
determinations of DNA methylation status, histone acetylation profiles, DNAse sensitivity,
transcription factor binding to DNA, levels of various proteins, messenger RNAs and miRNAs in
the cells of the marrow samples. By understanding how this process occurs in people without
blood diseases, we hope to to design future treatments for people with diseases such as
sickle cell disease and B-thalassemia.

Inclusion Criteria:

- Patients undergoing diagnostic bone marrow biopsies at the Norris Cotton Cancer Center
of DHMC.

- Patients undergoing bone marrow harvesting for autologous bone marrow transplantation.

- Normal donors undergoing bone marrow harvesting for autologous bone marrow

- Normal persons over the age of 17 who volunteer to undergo bone marrow aspiration.

Exclusion Criteria:

- Normal volunteers with known allergies to lidocaine (or similar analgesics)or
We found this trial at
1 Medical Center Dr
Lebanon, New Hampshire 03756
 (603) 650-5000
Principal Investigator: Christopher H Lowrey, MD
Dartmouth Hitchcock Medical Center Dartmouth-Hitchcock is a national leader in patient-centered health care and building...
Lebanon, NH
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