13C-Pyruvate Breath Test
| Status: | Completed |
|---|---|
| Conditions: | Healthy Studies |
| Therapuetic Areas: | Other |
| Healthy: | No |
| Age Range: | 21 - 50 |
| Updated: | 4/21/2016 |
| Start Date: | March 2013 |
| End Date: | July 2013 |
13C-Pyruvate Breath Test Pilot Study
The purpose of the test is to measure an enzyme called pyruvate. Subjects will fast for 12
hours and then undergo a breath test, have blood drawn, and cells from the inside of the
mouth collected over a 12 hour period. The tests will be repeated at least a week later and
subjects receive a dose of dichloroacetate (DCA)
hours and then undergo a breath test, have blood drawn, and cells from the inside of the
mouth collected over a 12 hour period. The tests will be repeated at least a week later and
subjects receive a dose of dichloroacetate (DCA)
Subjects will be screened with a history and physical and have baseline laboratory values
drawn to assure that the subject is healthy.Subjects will fast for 12 hours before the test.
The next day they will receive a dose of 25mg of pyruvate dissolved in 80ml of Crystal
Light. The pyruvate used in this study will be prepared with a nonradioactive form of
carbon, called a stable isotope. It poses no hazard to the subject's health. About 50 mls of
blood will be drawn over a 120 minute time period from an IV catheter placed in the arm.
Subjects will also be asked to exhale into a plastic tube about 12 times in 120 minutes to
collect 13C carbon dioxide (13CO2). Cells from inside of the mouth will be collected by
subjects swishing a tablespoon of mouth wash for one minute and then spitting it into a
sterile plastic tube. At least one week following the first admission subjects will return
to repeat the above procedure and receive 25mg of dichloroacetate (DCA) one hour before
receiving the pyruvate. Then the same samples will be collected. Subjects have the option of
doing the tests 4 times receiving the DCA on the last visit.
drawn to assure that the subject is healthy.Subjects will fast for 12 hours before the test.
The next day they will receive a dose of 25mg of pyruvate dissolved in 80ml of Crystal
Light. The pyruvate used in this study will be prepared with a nonradioactive form of
carbon, called a stable isotope. It poses no hazard to the subject's health. About 50 mls of
blood will be drawn over a 120 minute time period from an IV catheter placed in the arm.
Subjects will also be asked to exhale into a plastic tube about 12 times in 120 minutes to
collect 13C carbon dioxide (13CO2). Cells from inside of the mouth will be collected by
subjects swishing a tablespoon of mouth wash for one minute and then spitting it into a
sterile plastic tube. At least one week following the first admission subjects will return
to repeat the above procedure and receive 25mg of dichloroacetate (DCA) one hour before
receiving the pyruvate. Then the same samples will be collected. Subjects have the option of
doing the tests 4 times receiving the DCA on the last visit.
Inclusion Criteria:- healthy volunteers
- normal history,
- normal physical
- normal baseline laboratory data.
Exclusion Criteria:
- Any illness
- Chronic health condition
- Use of street drugs
- Taking medication
- Abnormal labs Abnormal physical exam -
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